{"id":199,"date":"2023-04-25T00:33:42","date_gmt":"2023-04-25T00:33:42","guid":{"rendered":"https:\/\/sites.wp.odu.edu\/geneticsspring2023\/?p=199"},"modified":"2023-04-25T00:33:42","modified_gmt":"2023-04-25T00:33:42","slug":"lac-operon-assignment","status":"publish","type":"post","link":"https:\/\/sites.wp.odu.edu\/geneticsspring2023\/2023\/04\/25\/lac-operon-assignment\/","title":{"rendered":"Lac Operon Assignment"},"content":{"rendered":"\n<p>Old Dominion University\/BIOL294-Genetics\/Rinehart-Kim\/Module 7\/Lac Operon Regulation<\/p>\n\n\n\n<p>Draw and describe the regulation of the Escherichia coli lac operon in the following situations:<\/p>\n\n\n\n<ol type=\"1\">\n<li>In the absence of lactose (disregard presence or absence of glucose).<\/li>\n<\/ol>\n\n\n\n<p>When there is no lactose (inducer) the repressor protein is bound to the operator, and the induction of lactose metabolism genes is repressed.<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><a href=\"https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-content\/uploads\/sites\/32818\/2023\/04\/image.png\"><img loading=\"lazy\" decoding=\"async\" width=\"909\" height=\"291\" src=\"https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-content\/uploads\/sites\/32818\/2023\/04\/image.png\" alt=\"\" class=\"wp-image-200\" srcset=\"https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-content\/uploads\/sites\/32818\/2023\/04\/image.png 909w, https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-content\/uploads\/sites\/32818\/2023\/04\/image-300x96.png 300w, https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-content\/uploads\/sites\/32818\/2023\/04\/image-768x246.png 768w\" sizes=\"(max-width: 909px) 100vw, 909px\" \/><\/a><\/figure>\n\n\n\n<ul>\n<li>In the presence of lactose (disregard presence or absence of glucose).<\/li>\n<\/ul>\n\n\n\n<p>When Lactose is present, the inducer binds to the repressor protein which changes its shape. This makes it so the repressor can no longer bind to the operator gene. RNA polymerase is now able to bind to the operator and promoter genes which also enables the transcription of the lacZ, lacY, and lacA genes.<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><a href=\"https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-content\/uploads\/sites\/32818\/2023\/04\/image-1.png\"><img loading=\"lazy\" decoding=\"async\" width=\"898\" height=\"548\" src=\"https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-content\/uploads\/sites\/32818\/2023\/04\/image-1.png\" alt=\"\" class=\"wp-image-201\" srcset=\"https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-content\/uploads\/sites\/32818\/2023\/04\/image-1.png 898w, https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-content\/uploads\/sites\/32818\/2023\/04\/image-1-300x183.png 300w, https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-content\/uploads\/sites\/32818\/2023\/04\/image-1-768x469.png 768w, https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-content\/uploads\/sites\/32818\/2023\/04\/image-1-665x406.png 665w\" sizes=\"(max-width: 898px) 100vw, 898px\" \/><\/a><\/figure>\n\n\n\n<ul>\n<li>In the absence of glucose (disregard the presence or absence of lactose).<\/li>\n<\/ul>\n\n\n\n<p>When there is no glucose, cAMP moves a catabolite activator protein (CAP) to the CAP site. This promotes the binding of RNA polymerase to the promoter and increases transcription.<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><a href=\"https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-content\/uploads\/sites\/32818\/2023\/04\/image-2.png\"><img loading=\"lazy\" decoding=\"async\" width=\"975\" height=\"401\" src=\"https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-content\/uploads\/sites\/32818\/2023\/04\/image-2.png\" alt=\"\" class=\"wp-image-202\" srcset=\"https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-content\/uploads\/sites\/32818\/2023\/04\/image-2.png 975w, https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-content\/uploads\/sites\/32818\/2023\/04\/image-2-300x123.png 300w, https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-content\/uploads\/sites\/32818\/2023\/04\/image-2-768x316.png 768w, https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-content\/uploads\/sites\/32818\/2023\/04\/image-2-912x375.png 912w\" sizes=\"(max-width: 975px) 100vw, 975px\" \/><\/a><\/figure>\n\n\n\n<ul>\n<li>In the absence of glucose AND the presence of lactose.<\/li>\n<\/ul>\n\n\n\n<p>When there is lactose and no glucose, the CAP\/cAMP is bound to the CAP site and the repressor is removed from the operator (due to the presence of an inducer). This allows the lac genes to be expressed and high amounts of transcription to occur.<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><a href=\"https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-content\/uploads\/sites\/32818\/2023\/04\/image-3.png\"><img loading=\"lazy\" decoding=\"async\" width=\"816\" height=\"485\" src=\"https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-content\/uploads\/sites\/32818\/2023\/04\/image-3.png\" alt=\"\" class=\"wp-image-203\" srcset=\"https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-content\/uploads\/sites\/32818\/2023\/04\/image-3.png 816w, https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-content\/uploads\/sites\/32818\/2023\/04\/image-3-300x178.png 300w, https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-content\/uploads\/sites\/32818\/2023\/04\/image-3-768x456.png 768w, https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-content\/uploads\/sites\/32818\/2023\/04\/image-3-683x406.png 683w\" sizes=\"(max-width: 816px) 100vw, 816px\" \/><\/a><\/figure>\n\n\n\n<p>Finally, describe where in the process of gene expression (transcription, post-transcription, translation, post-translation) this regulation takes place.<\/p>\n\n\n\n<p>The regulator is the repressor protein and regulation takes place at transcription. The repressor protein prevents the transcription process by physically blocking the RNA polymerase thus keeping the lac genes from being expressed. When the repressor is removed (via inducer) transcription begins and the genes are expressed.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Old Dominion University\/BIOL294-Genetics\/Rinehart-Kim\/Module 7\/Lac Operon Regulation Draw and describe the regulation of the Escherichia coli lac operon in the following situations: When there is no lactose (inducer) the repressor protein is bound to the operator, and the induction of lactose metabolism genes is repressed. When Lactose is present, the inducer binds to the repressor protein &hellip; <a href=\"https:\/\/sites.wp.odu.edu\/geneticsspring2023\/2023\/04\/25\/lac-operon-assignment\/\" class=\"more-link\">Continue reading <span class=\"screen-reader-text\">Lac Operon Assignment<\/span><\/a><\/p>\n","protected":false},"author":18617,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":true,"template":"","format":"standard","meta":{"footnotes":"","wds_primary_category":0},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-json\/wp\/v2\/posts\/199"}],"collection":[{"href":"https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-json\/wp\/v2\/users\/18617"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-json\/wp\/v2\/comments?post=199"}],"version-history":[{"count":1,"href":"https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-json\/wp\/v2\/posts\/199\/revisions"}],"predecessor-version":[{"id":204,"href":"https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-json\/wp\/v2\/posts\/199\/revisions\/204"}],"wp:attachment":[{"href":"https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-json\/wp\/v2\/media?parent=199"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-json\/wp\/v2\/categories?post=199"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.wp.odu.edu\/geneticsspring2023\/wp-json\/wp\/v2\/tags?post=199"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}