Oehler, J., Morrow, C. A., & Whitby, M. C. Gene duplication and deletion caused by over
\nreplication at a fork barrier. Nat. Commun. 14, 7730;
\nhttps:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC10676400\/ (2023).<\/p>\n
Oehler et al. hypothesized that stalling a replication fork at a specific locus during cellular DNA
\nreplication can induce gene duplication-deletion rearrangements. This duplication-deletion
\nrearrangement is characterized by the duplication of a specific gene to be added at a different
\nsite, while the original gene is deleted due to the whole duplication being moved.
\nExperimentally, they used a fission yeast, a strong polar replication fork barrier, a hygromycin
\nresistance gene, and a ura4 gene required for uracil synthesis. They placed the fork barrier
\n(RTS1) on chromosome 3 which replicates unidirectionally. Their experiment yielded a 100%
\nduplication-deletion rearrangement ratio of the correctly oriented RTS1, with 3 uniquely noted
\nclasses of dup-del.
\nThe research team then compared their work with RST1 to previous work of theirs that
\ndelayed fork convergence by deleting a strong centromere-proximal replication origin.
\nCombining the two methods provided an experimental ~6-fold increase in the RST1 induced
\nrecombinants with the ori deleted.
\nThey also found that downstream of RTS1, RDR can create a larger amount of replicated
\nDNA, with varying replicability. To determine how common such an occurrence is, they
\nmodified the experiment. The results of this showed that after the dup-del is initiated, the RDR
\nhas the capability of extending the leading strand of the collapsed replication fork beyond the
\ninitial barrier.<\/p>\n","protected":false},"excerpt":{"rendered":"
Oehler, J., Morrow, C. A., & Whitby, M. C. Gene duplication and deletion caused by over replication at a fork barrier. Nat. Commun. 14, 7730; https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC10676400\/ (2023). Oehler et al. hypothesized that stalling a replication fork at a specific locus during cellular DNA replication can induce gene duplication-deletion rearrangements. This duplication-deletion rearrangement is characterized by… <\/p>\n