{"id":228,"date":"2024-10-11T13:17:02","date_gmt":"2024-10-11T13:17:02","guid":{"rendered":"https:\/\/sites.wp.odu.edu\/jjohnson-engl110c\/?page_id=228"},"modified":"2024-10-13T23:35:53","modified_gmt":"2024-10-13T23:35:53","slug":"writing-assignment-4","status":"publish","type":"page","link":"https:\/\/sites.wp.odu.edu\/jjohnson-engl110c\/writing-assignment-4\/","title":{"rendered":"Writing Assignment #4"},"content":{"rendered":"\n<div class=\"wp-block-group is-vertical is-layout-flex wp-container-core-group-layout-1 wp-block-group-is-layout-flex\">\n<p>Jordan Johnson<\/p>\n\n\n\n<p>Professor Rinehart-Kim&nbsp;<\/p>\n\n\n\n<p>Bio. 294: Genetics&nbsp;<\/p>\n\n\n\n<p>09-16-2024<\/p>\n<\/div>\n\n\n\n<p class=\"has-text-align-center\">Summary of Primary Article\u00a0<\/p>\n\n\n\n<p>The research article \u201cThe functional role of inherited CDKN2A variants in childhood acute lymphoblastic leukemia\u201d focused on the coding variants of the CDKN2A gene, which is a tumor suppressor gene, and its association to acute lymphoblastic leukemia(ALL).\u00a0<\/p>\n\n\n\n<p>The first method they used to assess the coding variants of the CDKN2A gene was cytokine-independent growth assay. Cells were plated, washed, then plated at a specific density. Trypan blue was used to stain the cells on an automated cell counter, where viable cells were determined. Another method included a cell cycle assay, where the cells were treated with RNase A, stained, and analyzed by FACSCalibur. The final methods of analysis included a MTT-based cell toxicity assay, and a western blot.\u00a0<\/p>\n\n\n\n<p>The article revealed the impact of five CDKN2A variants on the susceptibility of ALL. This research can be used to guide the future of ALL treatment.<\/p>\n\n\n\n<div class=\"wp-block-group is-vertical is-layout-flex wp-container-core-group-layout-2 wp-block-group-is-layout-flex\">\n<p>Citation:&nbsp;<\/p>\n\n\n\n<p>Chunjie, L. et al.The functional role of inherited CDKN2A variants in childhood acute lymphoblastic leukemia.Pharmacogenet Genomics.Doi:10.1097\/FPC.0000000000000451.(2022)<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Jordan Johnson Professor Rinehart-Kim&nbsp; Bio. 294: Genetics&nbsp; 09-16-2024 Summary of Primary Article\u00a0 The research article \u201cThe functional role of inherited CDKN2A variants in childhood acute lymphoblastic leukemia\u201d focused on the coding variants of the CDKN2A gene, which is a tumor&#8230; <a class=\"more-link\" href=\"https:\/\/sites.wp.odu.edu\/jjohnson-engl110c\/writing-assignment-4\/\">Continue Reading &rarr;<\/a><\/p>\n","protected":false},"author":23371,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"_links":{"self":[{"href":"https:\/\/sites.wp.odu.edu\/jjohnson-engl110c\/wp-json\/wp\/v2\/pages\/228"}],"collection":[{"href":"https:\/\/sites.wp.odu.edu\/jjohnson-engl110c\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.wp.odu.edu\/jjohnson-engl110c\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.wp.odu.edu\/jjohnson-engl110c\/wp-json\/wp\/v2\/users\/23371"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.wp.odu.edu\/jjohnson-engl110c\/wp-json\/wp\/v2\/comments?post=228"}],"version-history":[{"count":3,"href":"https:\/\/sites.wp.odu.edu\/jjohnson-engl110c\/wp-json\/wp\/v2\/pages\/228\/revisions"}],"predecessor-version":[{"id":244,"href":"https:\/\/sites.wp.odu.edu\/jjohnson-engl110c\/wp-json\/wp\/v2\/pages\/228\/revisions\/244"}],"wp:attachment":[{"href":"https:\/\/sites.wp.odu.edu\/jjohnson-engl110c\/wp-json\/wp\/v2\/media?parent=228"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}