{"id":931,"date":"2020-07-13T16:47:56","date_gmt":"2020-07-13T16:47:56","guid":{"rendered":"https:\/\/sites.wp.odu.edu\/pakhomovlab\/?page_id=931"},"modified":"2020-07-13T17:07:15","modified_gmt":"2020-07-13T17:07:15","slug":"nanoelectroporation-by-nsep-identification-of-sensitive-ion-channels","status":"publish","type":"page","link":"https:\/\/sites.wp.odu.edu\/pakhomovlab\/nanoelectroporation-by-nsep-identification-of-sensitive-ion-channels\/","title":{"rendered":"Nanoelectroporation by nsEP: Identification of Sensitive Ion Channels"},"content":{"rendered":"\n<hr class=\"wp-block-separator\" \/>\n\n\n\n<p class=\"has-medium-font-size\">Cell membranes permeabilized by nsEP become permeable to small ions and water. This effect is explained the opening nanometer-size pores. However, the longevity of these pores (minutes) and their complex conductive properties have not been explained. Similar to some endogenous ion channels, nanopores are voltage- and current-sensitive, inward rectifying, and ion-selective.<\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-content\/uploads\/sites\/19252\/2020\/07\/Channels-870x1024.png\" alt=\"\" class=\"wp-image-933\" width=\"487\" height=\"574\" srcset=\"https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-content\/uploads\/sites\/19252\/2020\/07\/Channels-870x1024.png 870w, https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-content\/uploads\/sites\/19252\/2020\/07\/Channels-255x300.png 255w, https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-content\/uploads\/sites\/19252\/2020\/07\/Channels-127x150.png 127w, https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-content\/uploads\/sites\/19252\/2020\/07\/Channels-768x904.png 768w, https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-content\/uploads\/sites\/19252\/2020\/07\/Channels-1304x1536.png 1304w, https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-content\/uploads\/sites\/19252\/2020\/07\/Channels-1739x2048.png 1739w, https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-content\/uploads\/sites\/19252\/2020\/07\/Channels-679x800.png 679w\" sizes=\"(max-width: 487px) 100vw, 487px\" \/><\/figure><\/div>\n\n\n\n<p class=\"has-medium-font-size\">We hypothesize that nanopores are not just openings in the lipid bilayer, but may in fact be complex structures involving ion channels or other membrane proteins altered or damaged by the strong electric field. This exploratory project attempts the identification of such proteins. More than 300 genes coding for ion channels will be evaluated utilizing the CRISPR for gene knock-out. We employ high-throughput nsEP exposure and screening by membrane permeability and viability assays.<\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-content\/uploads\/sites\/19252\/2020\/07\/channels2-1024x918.png\" alt=\"\" class=\"wp-image-942\" width=\"421\" height=\"377\" srcset=\"https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-content\/uploads\/sites\/19252\/2020\/07\/channels2-1024x918.png 1024w, https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-content\/uploads\/sites\/19252\/2020\/07\/channels2-300x269.png 300w, https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-content\/uploads\/sites\/19252\/2020\/07\/channels2-150x134.png 150w, https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-content\/uploads\/sites\/19252\/2020\/07\/channels2-768x688.png 768w, https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-content\/uploads\/sites\/19252\/2020\/07\/channels2-1536x1377.png 1536w, https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-content\/uploads\/sites\/19252\/2020\/07\/channels2-2048x1836.png 2048w, https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-content\/uploads\/sites\/19252\/2020\/07\/channels2-800x717.png 800w\" sizes=\"(max-width: 421px) 100vw, 421px\" \/><\/figure><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Cell membranes permeabilized by nsEP become permeable to small ions and water. This effect is explained the opening nanometer-size pores. However, the longevity of these pores (minutes) and their complex conductive properties have not been explained. Similar to some endogenous &hellip; <a href=\"https:\/\/sites.wp.odu.edu\/pakhomovlab\/nanoelectroporation-by-nsep-identification-of-sensitive-ion-channels\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":17520,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"full-width-page.php","meta":{"footnotes":""},"_links":{"self":[{"href":"https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-json\/wp\/v2\/pages\/931"}],"collection":[{"href":"https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-json\/wp\/v2\/users\/17520"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-json\/wp\/v2\/comments?post=931"}],"version-history":[{"count":6,"href":"https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-json\/wp\/v2\/pages\/931\/revisions"}],"predecessor-version":[{"id":960,"href":"https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-json\/wp\/v2\/pages\/931\/revisions\/960"}],"wp:attachment":[{"href":"https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-json\/wp\/v2\/media?parent=931"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}