{"id":946,"date":"2020-07-13T17:01:29","date_gmt":"2020-07-13T17:01:29","guid":{"rendered":"https:\/\/sites.wp.odu.edu\/pakhomovlab\/?page_id=946"},"modified":"2020-07-13T17:08:22","modified_gmt":"2020-07-13T17:08:22","slug":"activation-of-voltage-gated-calcium-channels-vgcc-by-nsep","status":"publish","type":"page","link":"https:\/\/sites.wp.odu.edu\/pakhomovlab\/activation-of-voltage-gated-calcium-channels-vgcc-by-nsep\/","title":{"rendered":"Activation of Voltage-Gated Calcium Channels (VGCC) by nsEP"},"content":{"rendered":"\n<hr class=\"wp-block-separator\" \/>\n\n\n\n<p class=\"has-medium-font-size\">Nanosecond pulses may be too brief to cause translocation of the voltage sensor of VGCC and channel opening. Instead, nsEP can electroporate the membrane, causing depolarization due the loss of the resting membrane potential, which will culminate in the VGCC opening.<\/p>\n\n\n\n<p class=\"has-medium-font-size\">This project aims to establish if VGCC can be activated by nsEP directly, without the concurrent membrane damage by electroporation.<\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-content\/uploads\/sites\/19252\/2020\/07\/Ca-channel.png\" alt=\"\" class=\"wp-image-947\" width=\"479\" height=\"329\" srcset=\"https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-content\/uploads\/sites\/19252\/2020\/07\/Ca-channel.png 494w, https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-content\/uploads\/sites\/19252\/2020\/07\/Ca-channel-300x206.png 300w, https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-content\/uploads\/sites\/19252\/2020\/07\/Ca-channel-150x103.png 150w\" sizes=\"(max-width: 479px) 100vw, 479px\" \/><figcaption>HEK293 cells do not express endogenous VGCC. We transfect them to express VGCC (CaV1.3, red marker) and test if the VGCC is functional by the entry of Ca<sup>2+<\/sup> (green) upon depolarization in KCl. Next, we compare activation and membrane permeabilization by nsEP in cells which do and do not express functional VGCC.<\/figcaption><\/figure><\/div>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-content\/uploads\/sites\/19252\/2020\/07\/Ca-channel2.png\" alt=\"\" class=\"wp-image-948\" width=\"482\" height=\"315\" srcset=\"https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-content\/uploads\/sites\/19252\/2020\/07\/Ca-channel2.png 628w, https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-content\/uploads\/sites\/19252\/2020\/07\/Ca-channel2-300x196.png 300w, https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-content\/uploads\/sites\/19252\/2020\/07\/Ca-channel2-150x98.png 150w\" sizes=\"(max-width: 482px) 100vw, 482px\" \/><figcaption>Olympus IX81 microscope equipped for image recording concurrently with nsEP exposure, drug and solution delivery, and patch-clamp data acquisition<\/figcaption><\/figure><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Nanosecond pulses may be too brief to cause translocation of the voltage sensor of VGCC and channel opening. Instead, nsEP can electroporate the membrane, causing depolarization due the loss of the resting membrane potential, which will culminate in the VGCC &hellip; <a href=\"https:\/\/sites.wp.odu.edu\/pakhomovlab\/activation-of-voltage-gated-calcium-channels-vgcc-by-nsep\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":17520,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"full-width-page.php","meta":{"footnotes":""},"_links":{"self":[{"href":"https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-json\/wp\/v2\/pages\/946"}],"collection":[{"href":"https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-json\/wp\/v2\/users\/17520"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-json\/wp\/v2\/comments?post=946"}],"version-history":[{"count":5,"href":"https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-json\/wp\/v2\/pages\/946\/revisions"}],"predecessor-version":[{"id":962,"href":"https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-json\/wp\/v2\/pages\/946\/revisions\/962"}],"wp:attachment":[{"href":"https:\/\/sites.wp.odu.edu\/pakhomovlab\/wp-json\/wp\/v2\/media?parent=946"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}